Ingredient Library

What’s been studied. And what hasn’t.

Every ingredient across the range, with the published human research that exists on each one — listed with the details that let you judge it yourself: who was studied, at what dose, for how long, and by what method.

This is a reading list, not a results page. Research on an individual ingredient belongs to that study — its dose, its participants, its design — and does not describe what any finished formula does. Where an ingredient has little direct human research behind it, or where the research sits in a different population or a different subject area than you might expect, that is stated on the ingredient rather than left out.

IgniCognition

6 ingredients

IgniCognition™

Six ingredients, all active. The brand states the capsule contains no fillers or binders.

Shop this formulaFrom $199.00

Choose any ingredient to see the published human research behind it — who was studied, at what dose, for how long.

  • Multiple human trials
  • Direct human study
  • Indirect or adjacent
  • No direct study found
Multiple trials

Also called CDP-choline

A compound the body makes on its own, present in every cell. It carries choline and cytidine — two components cells draw on to build and maintain their outer membranes. Nerve cells have unusually large membrane surfaces for their size, which is part of why citicoline recurs in neurological research.

Human trials typically use 250–500 mg per day over 8–12 weeks. The literature splits between healthy older adults and clinical populations, including stroke recovery and diagnosed cognitive decline.

The 2 studies

Citicoline and Memory Function in Healthy Older Adults: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial

Design
Randomized · Double-blind · Placebo-controlled
Participants
100 healthy adults, ages 50–85
Dose
500 mg per day
Duration
12 weeks
Published
The Journal of Nutrition, 2021
Read on PubMed →

Double-blind placebo-controlled study with citicoline in APOE genotyped Alzheimer’s disease patients: effects on cognitive performance, brain bioelectrical activity and cerebral perfusion

Design
Randomized · Double-blind · Placebo-controlled
Participants
Adults with mild-to-moderate Alzheimer’s disease
Dose
Not recorded in our source listing
Duration
Not recorded in our source listing
Published
Methods and Findings in Experimental and Clinical Pharmacology, 2000
Read on PubMed →

Browse all Citicoline research on PubMed

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Human trial

Also called choline alphoscerate, or L-alpha glycerylphosphorylcholine

Another choline-carrying compound, found naturally in the body in small amounts and in foods such as organ meats and dairy. It is water-soluble and passes into the brain more readily than simple choline salts, which is generally why it is chosen over them in research.

Trials run 400–1,200 mg per day. Most human research on this ingredient has been conducted in people already diagnosed with cognitive impairment rather than in healthy adults — worth holding onto when reading it.

The study

Efficacy and safety of choline alphoscerate for amnestic mild cognitive impairment: a randomized double-blind placebo-controlled trial

Design
Randomized · Double-blind · Placebo-controlled · Multicenter
Participants
100 adults with amnestic mild cognitive impairment
Dose
600 mg per day
Duration
12 weeks
Published
Alzheimer’s Research & Therapy, 2024
Read on PubMed →

Browse all Alpha-GPC research on PubMed

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Human trial

A phospholipid, usually derived from soy or sunflower lecithin

A fat molecule forming part of the structural wall of every cell, at unusually high concentrations in brain tissue where it makes up a meaningful share of membrane material. The body produces it; diet contributes a small additional amount.

Human studies commonly use 100–300 mg per day and have examined memory measures, attention, and the body’s stress-hormone response. Populations vary widely across the literature.

The study

The effect of phosphatidylserine administration on memory and symptoms of attention-deficit hyperactivity disorder: a randomised, double-blind, placebo-controlled clinical trial

Design
Randomized · Double-blind · Placebo-controlled
Participants
Participants with attention-deficit hyperactivity disorder
Dose
200 mg per day, soy-derived
Duration
2 months
Published
Journal of Human Nutrition and Dietetics, 2014
Read on PubMed →

Browse all Phosphatidylserine research on PubMed

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Combination only

Also called CoQ10, ubiquinone, or ubiquinol

A fat-soluble compound the body manufactures itself, concentrated inside mitochondria — the structures within cells that convert food into usable energy. Natural production declines with age, part of why it became one of the most researched supplement ingredients in existence.

The literature is enormous, spanning cardiovascular, mitochondrial, neurological and fatigue research across decades. The trial below tested CoQ10 alongside NADH rather than on its own, so it is not isolated-ingredient evidence. For the larger body of isolated work, the PubMed link is the better starting point.

The study

Effect of Dietary Coenzyme Q10 Plus NADH Supplementation on Fatigue Perception and Health-Related Quality of Life in Individuals with Myalgic Encephalomyelitis / Chronic Fatigue Syndrome: A Prospective, Randomized, Double-Blind, Placebo-Controlled Trial

Design
Randomized · Double-blind · Placebo-controlled
Participants
Adults with myalgic encephalomyelitis / chronic fatigue syndrome
Dose
200 mg CoQ10 with 20 mg NADH, daily
Duration
Not recorded in our source listing
Published
Nutrients, 2021
Note
Combination formula — findings cannot be attributed to either ingredient alone
Read on PubMed →

Browse all Coenzyme Q10 research on PubMed

See the formula this is in
Combination only

Reduced nicotinamide adenine dinucleotide

A coenzyme present in every living cell and central to energy production — one of the molecules shuttling electrons through the reactions that turn food into cellular fuel. Biologically it is not obscure; it is fundamental.

Human supplementation research is a smaller field. Studies exist in fatigue and Parkinson’s disease contexts, but many are small and several test NADH inside a combination product rather than alone. The trial below is one of those.

The study

Effect of Dietary Coenzyme Q10 Plus NADH Supplementation on Fatigue Perception and Health-Related Quality of Life in Individuals with Myalgic Encephalomyelitis / Chronic Fatigue Syndrome: A Prospective, Randomized, Double-Blind, Placebo-Controlled Trial

Design
Randomized · Double-blind · Placebo-controlled
Participants
Adults with myalgic encephalomyelitis / chronic fatigue syndrome
Dose
20 mg NADH with 200 mg CoQ10, daily
Duration
Not recorded in our source listing
Published
Nutrients, 2021
Note
Combination formula — findings cannot be attributed to either ingredient alone
Read on PubMed →

Browse all NADH research on PubMed

See the formula this is in
No direct study

Often abbreviated NALT

A modified form of the amino acid L-tyrosine, which the body uses as raw material for dopamine and norepinephrine — two chemical messengers involved in alertness and focus. The added acetyl group is intended to make the compound more soluble in water.

Direct human research on the N-acetyl form itself is sparse. The performance research usually cited here was conducted on plain L-tyrosine, and the two are not interchangeable as evidence without pharmacological work showing the acetylated form behaves the same way in the body. Rather than present tyrosine research under a tyrosine-derivative heading, we have left the table empty.

Browse all N-Acetyl-L-Tyrosine research on PubMed

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IgniLongevity

7 ingredients

IgniLongevity™

Seven ingredients. The most heavily researched set in the range — and the one where the evidence is most mixed.

Shop this formulaFrom $199.00

Choose any ingredient to see the published human research behind it — who was studied, at what dose, for how long.

  • Multiple human trials
  • Direct human study
  • Indirect or adjacent
  • No direct study found
Multiple trials

Abbreviated NR; a form of vitamin B3

A form of vitamin B3 that the body converts into NAD+, a coenzyme involved in energy metabolism and DNA repair. NAD+ levels fall with age, which is the premise most of this research is built on.

The human literature is unusually consistent on one point and unsettled on another: trials repeatedly show that oral NR raises NAD+ levels in the blood, while what that increase produces downstream remains under investigation. Several trials describe their cognitive outcomes as exploratory.

The 3 studies

Nicotinamide riboside is uniquely and orally bioavailable in mice and humans

Design
Pharmacokinetic / biomarker trial
Participants
Human volunteers
Dose
Not recorded in our source listing
Duration
Not recorded in our source listing
Published
Nature Communications, 2016
Read on PubMed →

Chronic nicotinamide riboside supplementation is well-tolerated and elevates NAD+ in healthy middle-aged and older adults

Design
Randomized · Double-blind · Placebo-controlled · Crossover
Participants
Healthy middle-aged and older adults
Dose
Not recorded in our source listing
Duration
Not recorded in our source listing
Published
Nature Communications, 2018
Read on PubMed →

A randomized placebo-controlled trial of nicotinamide riboside in older adults with mild cognitive impairment

Design
Randomized · Placebo-controlled · Pilot
Participants
Older adults with mild cognitive impairment
Dose
Not recorded in our source listing
Duration
Not recorded in our source listing
Published
GeroScience, 2023
Note
Cognitive and blood-flow outcomes reported as exploratory
Read on PubMed →

Browse all Nicotinamide Riboside research on PubMed

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Mixed findings

Including trans-resveratrol; a polyphenol from grapes and berries

A plant compound found in red grapes, berries and peanuts, studied since the 1990s for its effects on metabolism and blood vessel function. It has one of the deepest human trial records of any ingredient on this page.

It also has one of the most contested. Trials range from 28 days to 24 months across healthy adults, postmenopausal women, people with obesity and people with type 2 diabetes — and a substantial number report no measurable effect. Four of the ten studies we found describe null or mixed results. We have listed a spread rather than only the positive ones.

The 4 studies

Long-term effects of resveratrol on cognition, cerebrovascular function and cardio-metabolic markers in postmenopausal women: A 24-month randomised, double-blind, placebo-controlled, crossover study

Design
Randomized · Double-blind · Placebo-controlled · Crossover
Participants
Postmenopausal women
Dose
Not recorded in our source listing
Duration
24 months
Published
Clinical Nutrition, 2021
Read on PubMed →

Calorie restriction-like effects of 30 days of resveratrol supplementation on energy metabolism and metabolic profile in obese humans

Design
Randomized · Double-blind · Crossover
Participants
Adults with obesity
Dose
150 mg per day
Duration
30 days
Published
Cell Metabolism, 2011
Read on PubMed →

High-dose resveratrol supplementation in obese men: an investigator-initiated, randomized, placebo-controlled clinical trial of substrate metabolism, insulin sensitivity, and body composition

Design
Randomized · Placebo-controlled
Participants
Men with obesity
Dose
High dose
Duration
Not recorded in our source listing
Published
Diabetes, 2013
Note
Reported largely null effects on the metabolic outcomes measured
Read on PubMed →

Resveratrol supplementation does not improve metabolic function in nonobese women with normal glucose tolerance

Design
Randomized · Double-blind · Placebo-controlled
Participants
Nonobese women with normal glucose tolerance
Dose
Not recorded in our source listing
Duration
Not recorded in our source listing
Published
Cell Metabolism, 2012
Note
Reported no improvement in the metabolic outcomes measured
Read on PubMed →

6 further studies on this ingredient were found and are available through the link below.

Browse all Resveratrol research on PubMed

See the formula this is in
Multiple trials

Abbreviated GSH; a tripeptide of glutamine, cysteine and glycine

A small protein the body synthesises itself, involved in the cell’s handling of oxidative stress and in liver processing of compounds. Levels are measurable in blood and tissue, which makes it easier to study than most supplement ingredients.

The central open question in this literature is absorption: whether swallowed glutathione survives digestion intact and raises body stores. Several trials listed below were designed specifically to test that question rather than any downstream outcome.

The 3 studies

Randomized controlled trial of oral glutathione supplementation on body stores of glutathione

Design
Randomized · Double-blind · Placebo-controlled
Participants
Adult volunteers
Dose
250 or 1,000 mg per day
Duration
6 months
Published
European Journal of Nutrition, 2015
Read on PubMed →

Effects of oral glutathione supplementation on systemic oxidative stress biomarkers in human volunteers

Design
Randomized · Double-blind · Placebo-controlled
Participants
Human volunteers
Dose
Not recorded in our source listing
Duration
Not recorded in our source listing
Published
Journal of Alternative and Complementary Medicine, 2011
Read on PubMed →

A Targeted Metabolomic Assessment of Oral Glutathione Bioavailability and Safety in Humans: A Randomized Crossover Clinical Trial

Design
Randomized · Crossover · Pharmacokinetic
Participants
Human volunteers
Dose
Comparison of oral delivery forms
Duration
Not recorded in our source listing
Published
Antioxidants, 2026
Read on PubMed →

1 further study on this ingredient were found and are available through the link below.

Browse all Reduced Glutathione research on PubMed

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Meta-analyses

A plant alkaloid from goldenseal, barberry and related species

A bright yellow compound extracted from several plants, used in traditional medicine and now among the most trial-tested botanicals in existence. Its research base is large enough that the strongest evidence comes from meta-analyses pooling dozens of trials rather than from any single study.

One thing to read carefully: almost all of this research was conducted in people with type 2 diabetes, metabolic syndrome, or dyslipidaemia. It is clinical-population evidence, and several trials compare berberine directly against prescription metformin.

The 4 studies

Effects of berberine on blood glucose in patients with type 2 diabetes mellitus: a systematic literature review and a meta-analysis

Design
Systematic review · Meta-analysis
Participants
28 randomized controlled trials, 2,313 participants
Dose
Not recorded in our source listing
Duration
Not recorded in our source listing
Published
Endocrine Journal, 2019
Read on PubMed →

Overall and Sex-Specific Effect of Berberine on Glycemic and Insulin-Related Traits: a Systematic Review and Meta-Analysis of Randomized Controlled Trials

Design
Systematic review · Meta-analysis
Participants
20 randomized trials, 1,761 participants
Dose
Not recorded in our source listing
Duration
Not recorded in our source listing
Published
The Journal of Nutrition, 2023
Read on PubMed →

Treatment of type 2 diabetes and dyslipidemia with the natural plant alkaloid berberine

Design
Randomized · Placebo-controlled
Participants
116 patients with type 2 diabetes and dyslipidaemia
Dose
1 g per day
Duration
3 months
Published
The Journal of Clinical Endocrinology & Metabolism, 2008
Read on PubMed →

Efficacy and safety of berberine on the components of metabolic syndrome: a systematic review and meta-analysis of randomized placebo-controlled trials

Design
Systematic review · Meta-analysis
Participants
Placebo-controlled trials in metabolic syndrome
Dose
Not recorded in our source listing
Duration
Not recorded in our source listing
Published
Frontiers in Pharmacology, 2025
Read on PubMed →

2 further studies on this ingredient were found and are available through the link below.

Browse all Berberine research on PubMed

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Different context

Also called 5-MTHF; the active form of folate, vitamin B9

The form of folate the body can use directly, without the enzymatic conversion step that folic acid requires. Folate itself is a well-established essential nutrient with a deep nutritional literature.

The trial evidence for supplemental L-methylfolate specifically, however, sits in a different area entirely: it was developed and tested as an add-on treatment in major depression. We have listed it as what it is rather than reframing it as longevity research.

The study

L-methylfolate as adjunctive therapy for SSRI-resistant major depression: results of two randomized, double-blind, parallel-sequential trials

Design
Two randomized · Double-blind · Placebo-controlled trials
Participants
Adults with SSRI-resistant major depression
Dose
Not recorded in our source listing
Duration
Not recorded in our source listing
Published
The American Journal of Psychiatry, 2012
Note
Evidence is in adjunctive treatment of major depression, not in longevity or healthy-ageing outcomes
Read on PubMed →

Browse all L-Methylfolate research on PubMed

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Human trial

Abbreviated PQQ

A compound found in trace amounts in soil bacteria and in some foods, identified relatively recently compared with the other ingredients here. Most of what is known about how it works comes from laboratory and animal research.

Direct human trial evidence is thin by comparison — a small number of randomized studies, mostly in healthy volunteers at doses around 20 mg per day.

The study

Effect of Dietary Pyrroloquinoline Quinone Disodium Salt on Cognitive Function in Healthy Volunteers: A Randomized, Double-Blind, Placebo-Controlled, Parallel-Group Study

Design
Randomized · Double-blind · Placebo-controlled · Parallel-group
Participants
Healthy volunteers
Dose
21.5 mg per day, PQQ disodium salt
Duration
12 weeks
Published
Journal of the American College of Nutrition, 2021
Read on PubMed →

Browse all Pyrroloquinoline Quinone research on PubMed

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Indirect only

A purified saponin from Astragalus membranaceus root

A compound isolated from Astragalus root, studied in the context of telomeres — the protective sequences at the ends of chromosomes that shorten as cells divide.

The human studies here need a caveat stated up front. Both were conducted on TA-65, a commercial Astragalus-derived preparation, not on chemically isolated cycloastragenol. That is related evidence, not direct evidence, and we have not presented it as the latter.

The 2 studies

A Natural Product Telomerase Activator Lengthens Telomeres in Humans: A Randomized, Double Blind, and Placebo Controlled Study

Design
Randomized · Double-blind · Placebo-controlled
Participants
Adult participants
Dose
TA-65 preparation
Duration
Not recorded in our source listing
Published
Rejuvenation Research, 2016
Note
Tested an Astragalus-derived formulation, not isolated cycloastragenol
Read on PubMed →

Evaluation of an oral telomerase activator for early age-related macular degeneration — a pilot study

Design
Randomized · Double-blind · Placebo-controlled · Pilot
Participants
Adults with early age-related macular degeneration
Dose
TA-65 preparation
Duration
Not recorded in our source listing
Published
Clinical Ophthalmology, 2016
Note
Tested an Astragalus-derived formulation, not isolated cycloastragenol
Read on PubMed →

Browse all Cycloastragenol research on PubMed

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IgniREM Sleep

8 ingredients

IgniREM Sleep™

Eight ingredients. Three of them have little or no direct human sleep research behind them — noted where that applies.

Shop this formulaFrom $199.00

Choose any ingredient to see the published human research behind it — who was studied, at what dose, for how long.

  • Multiple human trials
  • Direct human study
  • Indirect or adjacent
  • No direct study found
Human trial

The smallest amino acid

The simplest of the amino acids, made by the body and present in most protein-containing foods. It acts as a signalling molecule in the nervous system as well as a building block.

The sleep research is small but direct: placebo-controlled work using around 3 g before bedtime, generally measuring next-day subjective ratings rather than sleep architecture itself.

The study

The effects of glycine on subjective daytime performance in partially sleep-restricted healthy volunteers

Design
Placebo-controlled · Crossover
Participants
Healthy volunteers under partial sleep restriction
Dose
3 g before bedtime
Duration
Not recorded in our source listing
Published
Frontiers in Neurology, 2012
Read on PubMed →

Browse all Glycine research on PubMed

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Multiple trials

An essential amino acid

An amino acid the body cannot make and must obtain from food. It is the raw material from which serotonin, and in turn melatonin, are produced.

This is the oldest research base on the page. Sleep-laboratory trials date to the 1970s and used doses of 1–3 g, mostly in people with insomnia rather than healthy sleepers. Older trial design conventions are worth bearing in mind when reading them.

The 3 studies

The insomnia of ‘sleeping in a strange place’: effects of l-tryptophane

Design
Clinical trial
Participants
42 healthy subjects
Dose
1 g and 3 g
Duration
Not recorded in our source listing
Published
Psychopharmacology, 1977
Read on PubMed →

Treatment of severe chronic insomnia with L-tryptophan: results of a double-blind cross-over study

Design
Double-blind · Crossover
Participants
39 participants with chronic insomnia
Dose
Not recorded in our source listing
Duration
Not recorded in our source listing
Published
Pharmacopsychiatry, 1987
Read on PubMed →

Tryptophan for the sleeping disorder and mental symptom of new-type drug dependence: A randomized, double-blind, placebo-controlled trial

Design
Randomized · Double-blind · Placebo-controlled
Participants
Detoxified individuals with drug dependence
Dose
1,000 mg per day
Duration
2 weeks
Published
Medicine, 2016
Read on PubMed →

1 further study on this ingredient were found and are available through the link below.

Browse all L-Tryptophan research on PubMed

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Human trial

Abbreviated 5-HTP

The intermediate compound the body produces from tryptophan on the way to making serotonin. Taking it directly skips one conversion step.

Direct human sleep research is limited to a small number of controlled trials. The most recent, in older adults, reported that any benefit was most apparent among participants who slept poorly at baseline.

The study

The impact of 5-hydroxytryptophan supplementation on sleep quality and gut microbiota composition in older adults: A randomized controlled trial

Design
Randomized · Controlled
Participants
Older adults
Dose
Not recorded in our source listing
Duration
Not recorded in our source listing
Published
Clinical Nutrition, 2024
Note
Benefit most evident among poor sleepers at baseline
Read on PubMed →

Browse all 5-Hydroxytryptophan research on PubMed

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Human trial

Abbreviated GABA

The main inhibitory neurotransmitter in the brain — the signal that quiets neural activity rather than exciting it. Whether orally taken GABA reaches the brain in meaningful quantity is an open and actively debated question in the field.

The trial listed below is one of the few to use polysomnography, the sleep-laboratory measurement standard, rather than questionnaires alone.

The study

Safety and Efficacy of Gamma-Aminobutyric Acid from Fermented Rice Germ in Patients with Insomnia Symptoms: A Randomized, Double-Blind Trial

Design
Randomized · Double-blind · Placebo-controlled · Polysomnography
Participants
Patients with insomnia symptoms
Dose
300 mg per day
Duration
4 weeks
Published
Journal of Clinical Neurology, 2018
Read on PubMed →

Browse all Gamma-Aminobutyric Acid research on PubMed

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Adjacent only

Ascorbic acid

An essential nutrient with one of the largest research literatures in nutrition science, spanning a century.

Almost none of it addresses sleep directly. What exists is either observational — associations drawn from large population datasets — or trials in specific clinical conditions where sleep was a secondary consideration. Both listed studies are labelled accordingly.

The 2 studies

The Association Between Dietary Vitamin C and Sleep Disorders: A Cohort Study Based on UK Biobank

Design
Prospective cohort · Observational
Participants
UK Biobank cohort
Dose
Dietary intake, not supplementation
Duration
Not recorded in our source listing
Published
Nutrients, 2024
Note
An association study, not a supplementation trial — it cannot establish cause
Read on PubMed →

Efficacy of vitamins C, E, and their combination for treatment of restless legs syndrome in hemodialysis patients: a randomized, double-blind, placebo-controlled trial

Design
Randomized · Double-blind · Placebo-controlled
Participants
Haemodialysis patients with restless legs syndrome
Dose
Not recorded in our source listing
Duration
Not recorded in our source listing
Published
Sleep Medicine, 2012
Note
Relevance to general sleep quality is indirect
Read on PubMed →

Browse all Vitamin C research on PubMed

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Outside sleep

A flavonoid from Chinese skullcap, Scutellaria baicalensis

A plant compound from the root of Chinese skullcap, with a substantial laboratory literature examining how it interacts with receptors in the nervous system.

The human trial evidence, though, is in a different area. The randomized study below examined blood lipids and inflammation, not sleep. Sleep claims for baicalin rest largely on preclinical work. Baicalein, the closely related aglycone form, is a chemically distinct molecule and its research is not substituted here.

The study

Baicalin reduces blood lipids and inflammation in patients with coronary artery disease and rheumatoid arthritis: a randomized, double-blind, placebo-controlled trial

Design
Randomized · Double-blind · Placebo-controlled
Participants
Patients with coronary artery disease and rheumatoid arthritis
Dose
Not recorded in our source listing
Duration
Not recorded in our source listing
Published
Lipids in Health and Disease, 2018
Note
Direct human baicalin evidence, but not a sleep trial
Read on PubMed →

Browse all Baicalin research on PubMed

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No direct study

An amino sulfonic acid

A sulphur-containing compound found at high concentrations in the heart, muscle and nervous system. The body makes it, and it is present in meat and seafood.

We found no robust randomized trial testing isolated taurine specifically as a sleep intervention. The sleep-related literature that exists is largely observational, mechanistic, conducted in animals, or confounded by energy-drink formulations where taurine appears alongside caffeine — which makes attribution impossible.

Browse all Taurine research on PubMed

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No direct study

A bioflavonoid, most familiar from chamomile

A flavonoid present in chamomile, parsley and celery, studied in the laboratory for its interaction with receptors involved in sedation.

Direct human trials of isolated apigenin for sleep are sparse. Chamomile studies are frequently cited in its place, but whole chamomile contains many compounds and is not equivalent evidence for apigenin alone. We have not presented it as such.

Browse all Apigenin research on PubMed

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IgniPeptide Eye Serum

10 ingredients

IgniPeptide Eye Serum™

Seven actives plus a three-part preservation system. A topical cosmetic — the research here concerns the appearance and condition of skin.

Shop this formulaFrom $199.00

Choose any ingredient to see the published human research behind it — who was studied, at what dose, for how long.

  • Multiple human trials
  • Direct human study
  • Indirect or adjacent
  • No direct study found
Pilot studies

Abbreviated EGF

A protein the body produces as a signalling molecule in skin. It is among the most examined growth factors in dermatology research.

The topical human literature is real but modest in scale — pilot studies in the range of 18 to 50 participants, typically over 12 weeks. Two of the four studies listed tested multi-ingredient growth-factor formulations rather than EGF alone.

The 4 studies

Reduced appearance of under-eye bags with twice-daily application of epidermal growth factor (EGF) serum: a pilot study

Design
Pilot study · Topical
Participants
18 volunteers with under-eye bags
Dose
Twice-daily application
Duration
12 weeks
Published
Journal of Drugs in Dermatology, 2015
Read on PubMed →

A Prospective, Randomized, Double-blind, Split-face Clinical Trial Comparing the Efficacy of Two Topical Human Growth Factors for the Rejuvenation of the Aging Face

Design
Randomized · Double-blind · Split-face
Participants
Adults, facial application
Dose
Topical growth-factor formulations
Duration
Not recorded in our source listing
Published
The Journal of Clinical and Aesthetic Dermatology, 2017
Note
Tested growth-factor formulations, not isolated EGF
Read on PubMed →

Improvement in Atrophic Acne Scars Using Topical Synthetic Epidermal Growth Factor (EGF) Serum: A Pilot Study

Design
Pilot study · Topical
Participants
Adults with atrophic acne scars
Dose
Topical synthetic EGF serum
Duration
12 weeks
Published
Journal of Drugs in Dermatology, 2015
Read on PubMed →

Improvement in skin wrinkles using a preparation containing human growth factors and hyaluronic acid serum

Design
Clinical trial · Topical
Participants
Adults
Dose
Growth factors with hyaluronic acid
Duration
Not recorded in our source listing
Published
Journal of Cosmetic and Laser Therapy, 2015
Note
Combination formulation — findings cannot be attributed to EGF alone
Read on PubMed →

Browse all Epidermal Growth Factor research on PubMed

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Indirect only

Fermented marine algae extract

A marine extract produced by fermenting kelp, used in skincare as a source of minerals and amino acids.

Published human studies generally examine non-fermented seaweed extracts. Because a bioferment’s composition depends on the specific organism and fermentation process used, generic kelp-extract research is related rather than direct evidence for any particular bioferment.

The study

Effects of seaweed Laminaria japonica extracts on skin moisturizing activity in vivo

Design
Human topical study
Participants
Human volunteers
Dose
Laminaria japonica extract
Duration
Not recorded in our source listing
Published
Journal of Cosmetic Science, 2013
Note
Seaweed extract evidence, not evidence for a specific fermented bioferment
Read on PubMed →

Browse all Sea Kelp Bioferment research on PubMed

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Routes differ

Collagen broken into shorter peptide fragments

Collagen enzymatically cut into smaller fragments so that it can be formulated at a molecular weight low enough to interact with the skin surface.

Most published collagen research studies oral supplements rather than topical application. We have listed one trial that included a topical arm and one oral trial, labelled by route, because that distinction changes what the finding can be said to support.

The 2 studies

Efficacy and Safety of Topical or Oral Hydrolyzed Collagen in Women with Dermatoporosis: A Randomized, Double-Blind, Factorial Design Study

Design
Randomized · Double-blind · Factorial
Participants
Women with dermatoporosis
Dose
Topical and oral arms
Duration
Not recorded in our source listing
Published
Dermatology and Therapy, 2023
Read on PubMed →

Evaluation of the Efficacy of a Hydrolyzed Collagen Supplement for Improving Skin Moisturization, Smoothness, and Wrinkles

Design
Randomized · Controlled
Participants
Adults
Dose
Oral supplement
Duration
Not recorded in our source listing
Published
The Journal of Clinical and Aesthetic Dermatology, 2022
Note
Oral route — differs from topical serum use
Read on PubMed →

Browse all Hydrolyzed Collagen research on PubMed

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Mostly oral

Also called hyaluronan

A molecule naturally present in skin that binds and holds water, used as a humectant across the whole of skincare.

A pattern worth noticing in this literature: three of the four studies we found tested oral hyaluronan, not topical. The topical study listed first is the one conducted on the periocular area — the same region this product is formulated for.

The 2 studies

Efficacy of cream-based novel formulations of hyaluronic acid of different molecular weights in anti-wrinkle treatment

Design
Topical · Periocular application
Participants
76 women
Dose
0.1% hyaluronic acid, varying molecular weights
Duration
60 days
Published
Journal of Drugs in Dermatology, 2011
Read on PubMed →

Oral Hyaluronan Relieves Wrinkles and Improves Dry Skin: A 12-Week Double-Blinded, Placebo-Controlled Study

Design
Randomized · Double-blind · Placebo-controlled
Participants
Adults
Dose
Oral hyaluronan
Duration
12 weeks
Published
Nutrients, 2021
Note
Oral route — differs from topical serum use
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2 further studies on this ingredient were found and are available through the link below.

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Human trial

Also called glycerol

One of the oldest and most thoroughly established humectants in dermatology, drawing water toward the skin surface.

The trial listed is a large one by cosmetic-ingredient standards, though it studied a 20% cream in patients with eczema — a considerably higher concentration and a different context than a serum.

The study

A double-blind study comparing the effect of glycerin and urea on dry, eczematous skin in atopic patients

Design
Randomized · Double-blind
Participants
197 patients with atopic dry skin
Dose
20% glycerin cream
Duration
Not recorded in our source listing
Published
Acta Dermato-Venereologica, 2002
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Indirect only

Sodium pyrrolidone carboxylic acid

A component of the skin’s own natural moisturizing factor, the mixture that holds water in the outermost layer.

Controlled studies of sodium PCA in isolation are limited. It appears in clinical moisturizer research almost exclusively as one ingredient among many, which means its individual contribution cannot be separated out.

The study

A pilot study investigating the efficacy of botanical anti-inflammatory agents in an OTC eczema therapy

Design
Open-label · Pilot
Participants
Adults using an OTC eczema regimen
Dose
Multi-ingredient moisturizer containing sodium PCA
Duration
Not recorded in our source listing
Published
Journal of Cosmetic Dermatology, 2016
Note
Not isolated sodium PCA evidence
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Systematic reviews

From Aloe barbadensis leaf

A plant extract with three thousand years of topical use and, unusually for a botanical, a modern evidence base substantial enough to support several systematic reviews.

The research concentrates on wound healing, radiation dermatitis and psoriasis rather than cosmetic use around the eye. An umbrella review of that body of work found evidence strength varied considerably by indication.

The 3 studies

Aloe vera for prevention of radiation-induced dermatitis: A systematic review and cumulative analysis of randomized controlled trials

Design
Systematic review · Cumulative analysis
Participants
14 randomized controlled trials
Dose
Not recorded in our source listing
Duration
Not recorded in our source listing
Published
Frontiers in Public Health, 2022
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Management of psoriasis with Aloe vera extract in a hydrophilic cream: a placebo-controlled, double-blind study

Design
Randomized · Double-blind · Placebo-controlled · Topical
Participants
Patients with psoriasis
Dose
Aloe vera extract in hydrophilic cream
Duration
Not recorded in our source listing
Published
Tropical Medicine & International Health, 1996
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Aloe vera and health outcomes: An umbrella review of systematic reviews and meta-analyses

Design
Umbrella review
Participants
Systematic reviews and meta-analyses
Dose
Not recorded in our source listing
Duration
Not recorded in our source listing
Published
Phytotherapy Research, 2021
Note
Evidence strength varied by indication
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2 further studies on this ingredient were found and are available through the link below.

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Preservation System

Three ingredients present to keep the formula stable and safe rather than to act on skin. Their literature is regulatory and toxicological — safety assessment, not efficacy — and is listed on that basis.

Safety data

A 1,2-glycol; preservative booster and humectant

The study

Safety assessment of 1,2-glycols as used in cosmetics

Design
Cosmetic Ingredient Review safety assessment
Participants
Review of cosmetic use data
Dose
Not recorded in our source listing
Duration
Not recorded in our source listing
Published
International Journal of Toxicology, 2012
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Safety data

A broad-spectrum cosmetic preservative

The 2 studies

Opinion of the Scientific Committee on Consumer Safety (SCCS) — Final version of the opinion on Phenoxyethanol in cosmetic products

Design
Regulatory scientific safety opinion
Participants
EU Scientific Committee on Consumer Safety
Dose
Concluded safe at a maximum of 1.0%
Duration
Not recorded in our source listing
Published
Regulatory Toxicology and Pharmacology, 2016
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Safety review of phenoxyethanol when used as a preservative in cosmetics

Design
Review · Toxicology, exposure and sensitization
Participants
Published evidence review
Dose
Not recorded in our source listing
Duration
Not recorded in our source listing
Published
Journal of the European Academy of Dermatology and Venereology, 2020
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No direct study

A preservative also widely used in food

The indexed literature here is dominated by preservative performance, sensitization and toxicology rather than any effect on skin. We have not selected a curated study because there is no efficacy question to answer — the ingredient is present to keep the formula safe.

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